TC-5300/SIS-4M Binocular Inverted Brightfield/Phase Contrast Micromanipulator Injection Microscope System

The TC-5300/SIS-4M and TC-5400/SIS-4M Series are equipped with micromanipulators used to physically interact with a sample under a microscope, where a level of precision of movement is necessary that cannot be achieved by the unaided human hand. It consist of an input joystick, a mechanism for reducing the range of movement and an output section with the means of holding a microtool to hold, inject, cut or otherwise manipulate the object as required. The mechanism for reducing the movement is free of backlash. This is achieved by the use of kinematic constraints to allow each part of the mechanism to move only in one or more chosen degrees of freedom, which achieves a high precision and repeatability of movement, usually at the expense of some absolute accuracy.

Movement reduction can be performed by mechanical levers, hydraulically using pistons of different diameters connected by tubing containing non-compressible fluid, using combinations of techniques in one instrument. Mechanisms with different ranges of movement or variable reduction ratio may be incorporated in one instrument to allow coarse and fine positioning.

Depending on the application, users may require different scales of movement resolution, movement speed, range and accuracy. These are the critical variables integrated into manipulator designed by manufacturers, which are typically presented to suit particular applications.

Our latest inverted microscope line is the cost-effective TC-5000 Series Inverted Biological Microscopes. The TC Series makes routine examining of tissue cultures, microbes and other live specimens in petri dishes and culture bottles fast and easy while delivering an excellent performance-to-cost ratio.

The TC Series is available in brightfield, phase contrast and epi-fluorescence models. The TC5000 Series Microscopes have an integrated front mounted camera port with adapters available for 35mm, CCD/CMOS and other cameras.

It is a special model combining Meiji Techno's inverted microscope TC series with Micromanipulator, injector etc which is the best match for TC series by S Corporation. Three-dimensional hydraulic micromanipulator is driving distance X / Y / Z axes 20mm and, smooth movement of the hydraulic and feature a. Pneumatic injector is unnecessary filling of oil yet, it achieved a response of more than oil formula was. It is with a push type air refill valve. The injection holder is equipped with a pipette stopper and a positioning stopper. The adapter part with the microscope is equipped with a bounce lever . By tilting the lever, the entire left and right driving part jumps up, and it is possible to easily exchange the capillary pipette and replace the sample. It is a high performance yet simple and easy to use injection system set. It adopts a special specification for the lens barrel of theinverted microscope and it is possible to observe an erect image, making it ideal for micromanipulation.

(Capillary pipette and dish are not included in the product)


Made In Japan


Locate a DealerGet a Quote

The TC-5300/SIS-4M & TC-5400/SIS-4M Series are suitable for viewing colorless and transparent specimens and live cells. The TC-5300/SIS-4M & TC-5400/SIS-4M Series is a contrast-enhancing optical technique that can be utilized to produce high-contrast images of transparent specimens such as living cells, microorganisms, thin tissue slices, lithographic patterns, and sub-cellular particles (such as nuclei and other organelles). In effect, the phase contrast technique employs an optical mechanism to translate minute variations in phase into corresponding changes in amplitude, which can be visualized as differences in image contrast. One of the major advantages of phase contrast microscopy is that living cells can be examined in their natural state without being killed, fixed, and stained. As a result, the dynamics of ongoing biological processes in live cells can be observed and recorded in high contrast with sharp clarity of minute specimen detail.

Includes:

   • TC-5300/SIS-4M: Binocular Inverted Phase Contrast/Brightfield Compound Microscope, ∞ Infinity Corrected Optical System F-200MM with 6V 30W Halogen Koehler illumination with quintuple nosepiece and auto voltage sensing power supply, with SIS-4M-U Micromanipulator Injector, Adaptor and Manipulator (Drive Unit), Main Control unit, Universal Joint (with Coarse movement remote-control type), Iron plate
   • MA814: Siedentopf type binocular, inclined at 30°, Interpupillary adjustment 53mm to 75mm graduated diopter on left eyetube (30mm I.D. eyetubes)
   • MA817 (x 2): SWH10X Widefield high eyepoint eyepiece, Field No. 22, tube O.D. 30.0 mm
   • MA825: TC Long Working Distance LWD Planachromat Phase 4X objective ∞/1.0, W.D. 17.3 mm
   • MA826: TC Long Working Distance LWD Planachromat Phase 10X objective ∞/1.0, W.D. 7.6 mm
   • MA827: TC Long Working Distance LWD Planachromat Phase 20X objective ∞/1.0, W.D. 7.0 mm
   • MA828: TC Long Working Distance LWD Planachromat Phase 40X objective ∞/1.0, W.D. 2.8 mm
   • MA853: TC Extra Long Working Distance  ELWD Condenser, N.A. 0.30, W.D. 73.0mm
   • MA861: Green interference filter in 51mm mount (546mm)
   • MA863: Cobalt clear filter in mount in 51mm mount
   • STAGE: Plain stage with 180mm(X) x 245mm(Y) plain stage with replaceable glass insert with 45mm opening
   • MA878: Glass Stage plate insert for plain stage (Round-109mm diameter, 1.5mm thick with 44.3mm central opening)
   • MA855: Phase slider
   • MA458/05: Centering telescope (O.D. = 30.0)
   • MA809/10: Removable AC Electric cord with plug for 115V models 
   • MA326: Bulb 6V 30W Halogen (Factory Installed)
   • MA327: Fuse 3 Amp (Factory Installed)
   • Manual: TC-5000 Meiji Techno Instruction Manual
   • Warranty: Limited Lifetime Warranty Card 
   • SIS-4M-U: Micromanipulator Injector, Adaptor and Manipulator (Drive Unit), Main Control unit, Universal Joint (with Coarse movement remote-control type), Iron plate

Injection System Set

Set configuration
   • Two 3D hydraulic micromanipulators
   • Two iron plates for fixing the joystick
   • Two sets of injectors
   • Two universal joints
   • One TC seres connection adapter
Manipulator specification
Overview    • Hydraulic three-dimensional manipulator with smooth movement
   • Drive distance of 20 mm realized
   • Compact drive unit
Driving distance X/Y/Z Each axis 20 mm (one turn of handle 1mm)
Maximum driving distance on the joystick XY plane 2 mm
Injector specification
Overview    • Because it is a pneumatic type, oil filling is unnecessary
   • Response more than oil type realized
   • Work efficiency with push type air replenishing valve UP
Working distance (universal joint) 15 mm
Remarks Injection holder (with pipette stopper positioning stopper TC series connection adapter (with splash function) Universal joint

 

Product Information:
   • Manufactured: 100% MADE IN JAPAN
   • Warranty: LIMITED LIFETIME WARRANTY
   • Customer Support: 1-(800) 832-0060 (U.S.A Based)

Package Information:
   • Weight: 23 lbs (10.5 kg)
   • Box Size: 16 x 14 x 21 inches
   • Footprint: 364mm (D) x 220mm (W)
   • Binocular head type: 485 mm(D) x 411 mm(H) x 220 mm(W) (8.0kg)( 29.0 lbs)
   • Trinocular head type: 485 mm(D) x 440 mm(H) x 2210 mm(W) (8.5Kg)(31.0 lbs)
   • Binocular Ergonomic head type: 485 mm(D) x 440mm(H) x 2210mm(W) (8.5Kg)(31.0 lbs)

Model
Head
Illumination
Eyepieces
Objectives
Stage
Condenser

TC-5100 Brightfield

Binocular
Koehler w/o iris 6V 30W halogen
SWH10x FN22
Quintuple Nosepiece w/LWD 10X &
LWD 20X
Plain Fixed Stage
180mm X 247mm
TC Condenser 
WD 73mm
NA 0.30orTC Condenser2 WD 20.5mm
NA 0.55

TC-5100E Brightfield

Ergonomic Binocular

TC-5200 Brightfield

Trinocular

TC-5300 Phase Contrast

Binocular
Koehler w/o iris
with phase slider 6V 30W halogenKoehler 6V 30W halogen

TC-5300E Phase Contrast

Ergonomic Binocular

TC-5400 Phase Contrast

Trinocular

TC-5500 Epi-Fluorescent

Binocular 100W Mercury + Koehler w/o iris   6V 30W halogen

Quintuple Nosepiece
TC Planachromat
F10X, F20X

TC Condenser WD 73mm
NA 0.30 or TC Condenser2 WD 20.5mm
NA 0.55

TC-5600 Epi-Fluorescent

Trinocular

VIEWING HEADS
Binocular models, the TC5100, TC5300 and TC5500, incorporate the MA814 Siedentopf-type binocular head while the trinocular models, the TC5200, TC5400 and TC5600 have the MA816/10 Siedentopf-type trinocular head. The MA816/10 uses an 80/20 beamsplitter that can be engaged for photo-microscopy. (100% to eyetubes or 80% to phototube and 20% to the eyetube.) Each microscope head has the eyetubes inclined at 30 degrees with the left eyetube having graduated diopter settings. The interpupillary distance on the viewing heads is adjustable between 53mm - 75mm. 

EYEPIECES
10X Super Widefield High Eyepoint eyepieces are standard and 15X and 20X eyepieces are also available as an option. A Super Widefield High Eyepoint 10X focusable eyepiece that accepts standard 25mm reticules is also available.

OBJECTIVE CHANGER
Quintuple nosepiece provides effortless objective changes with smooth-operating, ball bearing mounted.

OBJECTIVES
Meiji Techno America offers an assortment of Planachromat Infinity Corrected (ICOS) objectives for Brightfield, Darkfield and Phase Contrast observation modes.

STAGE
The optional attachable mechanical stage measures 112mm(X) x 72mm(Y). It has ergonomically positioned right-handed coaxial drop down controls. It also accepts the following optional accessory plates: 35mm, 55mm, 65mm, Petrie Dish Holders, 96-well Terasaki Plate Holder, 1" X 3" glass slide holder, Hemacytometer holder.Slide, chamber, and petri dish holding plates of various sizes are available separately.  

CONDENSER
The standard condenser has a working distance of 73mm.

ILLUMINATION
A powerful 6 volt 30 watt halogen bulb with pre-centered socket provides enhanced image quality and brightness for the observation of specimens.

PHOTOMICROGRAPHY
Meiji Techno offers a broad range of digital video cameras which offers excellent sensitivity, high resolution and a wide dynamic range for the most demanding brightfield and darkfield microscopy applications including clinical pathology and cytology, life sciences and geology. Some models has included software, that can preview and capture still shots and live videos to a PC or Mac with USB port or HDMI connectivity.

Details

The TC-5300/SIS-4M and TC-5400/SIS-4M Series are equipped with micromanipulators used to physically interact with a sample under a microscope, where a level of precision of movement is necessary that cannot be achieved by the unaided human hand. It consist of an input joystick, a mechanism for reducing the range of movement and an output section with the means of holding a microtool to hold, inject, cut or otherwise manipulate the object as required. The mechanism for reducing the movement is free of backlash. This is achieved by the use of kinematic constraints to allow each part of the mechanism to move only in one or more chosen degrees of freedom, which achieves a high precision and repeatability of movement, usually at the expense of some absolute accuracy.

Movement reduction can be performed by mechanical levers, hydraulically using pistons of different diameters connected by tubing containing non-compressible fluid, using combinations of techniques in one instrument. Mechanisms with different ranges of movement or variable reduction ratio may be incorporated in one instrument to allow coarse and fine positioning.

Depending on the application, users may require different scales of movement resolution, movement speed, range and accuracy. These are the critical variables integrated into manipulator designed by manufacturers, which are typically presented to suit particular applications.

The TC-5000 Brightfield/Phase Contrast Series features include:

  • Seidentopf type binocular head inclined at 30° or trinocular at 45°
  • Equipped with 6V, 30W Halogen lamp illuminator
  • N.A. 0.30 condenser
  • 180mm(X) X 245mm(Y) stage with replaceable glass insert with 45mm opening
  • LWD objectives are Phase 4X, 10X, 20X, and 40X
  • SIS-4M-U: Micromanipulator Injector, Adaptor and Manipulator (Drive Unit), Main Control unit, Universal Joint (with Coarse movement remote-control type), Iron plate
  • Exchangeable observation tubes
  • Integrated Automatic Voltage Sensing Power Supply with localized power cord
  • Compact wedge shaped cast aluminum alloy frame for exceptional stability and a small footprint
  • Chemical resistant baked enamel finish
  • Flexible and modular design for system expansion
  • Transparent stage plate for easy objective identification
  • Low profile coaxial and fine focus controls with tension adjustment
  • Precentered 30W Halogen bulb
  • Front mounted CCD, analog or SLR cameras
  • Integrated Automatic Voltage Sensing Power Supply with localized power cord
  • Optional mechanical stage with specimen holders

Brightfield:
The simplest of all the optical microscopy illumination techniques. Sample illumination is transmitted (i.e., illuminated from below and observed from above) white light and contrast in the sample is caused by absorbance of some of the transmitted light in dense areas of the sample. Brightfield microscopy is the simplest of a range of techniques used for illumination of samples in light microscopes and its simplicity makes it a popular technique. The typical appearance of a brightfield microscopy image is a dark sample on a bright background.

Phase Contrast:
An optical microscopy technique that converts phase shifts in light passing through a transparent specimen to brightness changes in the image. Phase shifts themselves are invisible, but become visible when shown as brightness variations. When light waves travel through a medium other than vacuum, interaction with the medium causes the wave amplitude and phase to change in a manner dependent on properties of the medium. Changes in amplitude (brightness) arise from the scattering and absorption of light, which is often wavelength-dependent and may give rise to colors. Photographic equipment and the human eye are only sensitive to amplitude variations. Without special arrangements, phase changes are therefore invisible.

The TC-5300 & TC-5400 Series are suitable for viewing colorless and transparent specimens and live cells. The TC-5300 & TC-5400 Series is a contrast-enhancing optical technique that can be utilized to produce high-contrast images of transparent specimens such as living cells, microorganisms, thin tissue slices, lithographic patterns, and sub-cellular particles (such as nuclei and other organelles). In effect, the phase contrast technique employs an optical mechanism to translate minute variations in phase into corresponding changes in amplitude, which can be visualized as differences in image contrast. One of the major advantages of phase contrast microscopy is that living cells can be examined in their natural state without being killed, fixed, and stained. As a result, the dynamics of ongoing biological processes in live cells can be observed and recorded in high contrast with sharp clarity of minute specimen detail.

Components & Accessories

Included:
   • TC-5300/SIS-4M: Binocular Inverted Phase Contrast/Brightfield Compound Microscope, ∞ Infinity Corrected Optical System F-200MM with 6V 30W Halogen Koehler illumination with quintuple nosepiece and auto voltage sensing power supply, with SIS-4M-U Micromanipulator Injector, Adaptor and Manipulator (Drive Unit), Main Control unit, Universal Joint (with Coarse movement remote-control type), Iron plate
   • MA814: Siedentopf type binocular, inclined at 30°, Interpupillary adjustment 53mm to 75mm graduated diopter on left eyetube (30mm I.D. eyetubes)
   • MA817 (x 2): SWH10X Widefield high eyepoint eyepiece, Field No. 22, tube O.D. 30.0 mm
   • MA825: TC Long Working Distance LWD Planachromat Phase 4X objective ∞/1.0, W.D. 17.3 mm
   • MA826: TC Long Working Distance LWD Planachromat Phase 10X objective ∞/1.0, W.D. 7.6 mm
   • MA827: TC Long Working Distance LWD Planachromat Phase 20X objective ∞/1.0, W.D. 7.0 mm
   • MA828: TC Long Working Distance LWD Planachromat Phase 40X objective ∞/1.0, W.D. 2.8 mm
   • SIS-4M-U: Micromanipulator Injector, Adaptor and Manipulator (Drive Unit), Main Control unit, Universal Joint (with Coarse movement remote-control type), Iron plate
   • MA853: TC Extra Long Working Distance  ELWD Condenser, N.A. 0.30, W.D. 73.0mm
   • MA861: Green interference filter in 51mm mount (546mm)
   • MA863: Cobalt clear filter in mount in 51mm mount
   • STAGE: Plain stage with 180mm(X) x 245mm(Y) plain stage with replaceable glass insert with 45mm opening
   • MA878: Glass Stage plate insert for plain stage (Round-109mm diameter, 1.5mm thick with 44.3mm central opening)
   • MA855: Phase slider
   • MA458/05: Centering telescope (O.D. = 30.0)
   • MA809/10: Removable AC Electric cord with plug for 115V models 
   • MA326: Bulb 6V 30W Halogen (Factory Installed)
   • MA327: Fuse 3 Amp (Factory Installed)
   • Manual: TC-5000 Meiji Techno Instruction Manual
   • Warranty: Limited Lifetime Warranty Card 

OPTIONAL COMPONENTS & ACCESSORIES BELOW

Viewing Heads:
   • MA816/10: Siedentopf type trinocular head, inclined at 30°, I.P. adjustment 53mm to 75mm graduated diopter on left eyetube (30mm I.D. eyetubes) (Included with TC-5400) Includes 80/20 beamsplitter (100% to eyetubes or 80% to photo 20% to eyetube)
   • MA957/15: Ergonomic binocular head, inclination adjustable vertically from 10° to 50° (Included with TC-5100E)
   • MA1034: Trinocular Siedentopf head, erect image, inclined at 30°, I.P. adjustment 53mm to 75mm, graduated diopter on left eyetube (30mm I.D. eyetubes) Includes 0/100 beamsplitter (100% to eyetubes or 100% to phototube)

Eyepieces:
   • MA818: SWH 15X Widefield high eyepoint eyepiece, Field No. 16.0, tube O.D. 30.0 mm (each) (19mm reticle mount)
   • MA819: SWH 20X Widefield high eyepoint eyepiece, Field No. 12.0, tube O.D. 30.0 mm (each) (19mm reticle mount)
   • MA859: SWH 10X-F Focusing eyepiece with reticle mount (without reticle), tube O.D. 30.0 mm (each) (25mm reticle mount)

Phase Objectives:
   • MA825: TC Planachromat Phase 4X objective ∞/1.0 N.A. 0.13, F.L. 50.0, W.D. 17.3
   • MA828: TC Planachromat Phase 40X objective ∞/1.0 N.A. 0.65, F.L. 5.0, W.D. 2.8

Condensers:
   • MA854: TC Condenser, N.A. 0.55, W.D. 20.5mm (For use with MA823 40X objective)

Filters:
   • MA856: LB100 Blue clear filter in 51mm mount
   • MA857: G533 Green clear filter in 51mm mount
   • MA858: ND25 Neutral density filter in 51mm mount

Miscellaneous:
   • MA809/20: Removable AC Electric cord with plug for 220V models (included in all 220V models) CAMERA ADAPTERS FOR “C” MOUNT TYPE CAMERAS
   • MA877: “C” mount type camera adapter for CCD/CMOS camera (No internal lens) (Magnification factor of 0.66X from TC5000 Series front camera port)
CAMERA ADAPTERS FOR DIGITAL STILL CAMERAS
   • MA868: Digital camera adapter for non “C” mount type cameras (52mm O.D. thread) For TC Series front camera port only: (Requires additional DCA adapter ring for your specific model of camera which are listed on the last two pages of the TC5000 section) CAMERA ADAPTERS FOR 35mm TYPE CAMERAS
   • MA869: 35mm SLR camera attachment Magnification at film plane is 2.23X of objective in use

Stages (Fixed 245mm x 180mm, plain stage is included with stand):
   • MA878: Replacement glass stage plate insert for plain stage - TC5000 Series (Round - 109mm diameter, 1.5mm thick with 44.3mm opening) (Fits plain stage only not for use with MA380/05)
   • MA380/05: Attachable mechanical stage, movement 112mm(X) x 72mm(Y) with test plate holder and drop-down coaxial X-Y controls.

COMPONENTS OFTEN PAIRED WITH THE MA380/05 MECHANICAL STAGE

Specimen Holders for use with MA380/05 Attachable Mechanical Stage (Rectangular Plate 129.74mm x 89.78 mm x 3mm):
   • MA382: Slide glass holder, 1”x 3” 
   • MA383: Chamber holder (For 55mm x 80mm chamber)
   • MA384: Petri dish holder 35mm diameter
   • MA385: Petri dish holder 55mm diameter
   • MA387: Petri dish holder 65mm diameter
   • MA864: Terasaki plate holder (For 56mm x 87mm Terasaki plate)
   • MA378: Hemacytometer holder

Stage Micrometers:
   • MA285: Objective micrometer, 0.01mm ruling
   • MA286: 0.04” divided into 40 units, 0.001
   • MA285: 1mm divided into 100 parts

Eyepiece Micrometers:
   • MA523: Cross-line reticule, 25mm diameter
   • MA506: Eyepiece micrometer, 10mm divided into 100 units, 25mm diameter
   • MA509: Eyepiece micrometer 5mm divided into 100 units, 25mm diameter
   • MA524: 10mm square divided into 100 parts, 1.0mm square, 25mm diameter
   • MA542: Cross-line with 0.1mm graduations, 21mm diameter


Double click to read at full screen
Download Brochure (PDF)



Double click to read at full screen
Download Manual (PDF)