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Embryo Collection

Embryo collection is the study of techniques and processes used to retrieve, handle, and preserve embryos for purposes such as assisted reproduction, genetic research, and conservation. This field encompasses methods for collecting embryos from humans or animals, ensuring their viability, and storing them for future use in fertility treatments or scientific studies.

Microscopes play a crucial role in embryo collection in both clinical settings (like IVF clinics) and research environments (such as developmental biology labs).

Here’s how they’re used at different stages:

 1. Oocyte (Egg) Identification and Retrieval

  • Meiji Techno EMZ Stereomicroscopes are used to visually identify and isolate mature oocytes (eggs) from ovarian follicles or aspirated fluids.
  • They allow embryologists to see eggs clearly in follicular fluid and separate them from debris.

 2. Insemination and Fertilization (e.g., IVF or ICSI)

  • Meiji Techno TC5000 Inverted microscopes are used for:
    • In Vitro Fertilization (IVF): Visualizing eggs and sperm interaction.
    • Intracytoplasmic Sperm Injection (ICSI): Directly injecting a single sperm into an egg under high magnification (usually 400x or higher).with micromanipulators.

 3. Embryo Assessment and Selection

  • Microscopes are used to monitor the development of embryos post-fertilization (cleavage, blastocyst formation).
  • Time-lapse imaging systems integrated with microscopes allow constant observation without disturbing embryo conditions.
  • Embryologists check:
    • Cell division timing
    • Cell symmetry
    • Fragmentation level
    • Blastocyst quality

 4. Embryo Biopsy and Genetic Testing

  • High-resolution microscopes assist in laser-assisted biopsy, where a few cells are removed from the embryo (for PGD/PGT) without damaging it.

 5. Embryo Transfer and Cryopreservation

  • Microscopes are used to confirm embryo integrity before transfer into the uterus or before/after vitrification (freezing).

Common Types of Microscopes Used:

Microscope Type Purpose
Stereo Microscope Egg identification, oocyte retrieval
Inverted Microscope Embryo culture monitoring, ICSI
Fluorescence Microscope Genetic screening (e.g., FISH)
Time-Lapse Microscopy Real-time embryo development tracking


Embryo Collection

UNIVERSITY

The following item requires an illumination:
EMZ-5 + MA502 + P Stand

  • EMZ-5 + MA502 + P, Stereo Zoom Binocular Stand Configuration System, Magnification (7X – 45X), W.D. 93mm (3.66″)

  • EMZ-5 + MA502 + PKL-2, Stereo Zoom Binocular Stand Configuration System, Magnification (7X – 45X), W.D. 93mm (3.66″)

  • EMZ-13 + MA502 + PLS-2, Stereo Zoom Binocular Stand Configuration System, Magnification (10X – 70X), W.D. 90mm (3.54″)

  • CZ-1105 1X CMO Binocular Zoom Stereo System, Working Distance 78mm

  • CZ-1105TR 1X CMO Trinocular Zoom Stereo System, Working Distance 78mm

  • CZ-1100 1X CMO Ergonomic Binocular Zoom Stereo System, Working Distance 78mm

  • CZ-1100TR 1X CMO Ergonomic Trinocular Zoom Stereo System, Working Distance 78mm

The following item requires an illumination:
EMZ-5 + MA502 + F + S-4100 Binocular
EMZ-13 + MA502 + FS + S-4300 Binocular

  • EMZ-13 + MA502 + FS + S-4300, Stereo Zoom Binocular Stand Configuration System, Magnification (10X – 70X), W.D. 90mm (3.54″)

  • EMZ-5H + MA522 + F + S-1100, Stereo Zoom Binocular Stand Configuration System, Magnification (7X – 45X), W.D. 93mm (3.66″)

  • EMZ-5 + MA502 + F + S-4100, Stereo Zoom Binocular Stand Configuration System, Magnification (7X – 45X), W.D. 93mm (3.66″)

  • EMZ-13TR + MA502 + F + S-4100 + MA961D/S/ESD + MA151/35/03 + HD1500TM, Stereo Zoom Trinocular Stand Configuration System, Magnification (1.0X – 7.0X), W.D. 90mm (3.54″)

The following item requires an illumination:
EMZ-5 + MA502 + F + FA-4 Binocular

  • EMZ-13 + MA502 + FSC + SAS-3, Stereo Zoom Binocular Stand Configuration System, Magnification (10X – 70X), W.D. 90mm (3.54″)

  • EMZ-5 + MA502 + F + FA-4, Stereo Zoom Binocular Stand Configuration System, Magnification (7X – 45X), W.D. 93mm (3.66″)

  • EMZ-5TRH + MA522 + CR-2, Stereo Zoom Trinocular Stand Configuration System, Magnification (7X – 45X), W.D. 93mm (3.66″)

  • EMZ-5TRH + MA522 + F + FA-4, Stereo Zoom Trinocular Stand Configuration System, Magnification (7X – 45X), W.D. 93mm (3.66″)

The following item requires an illumination:
EMZ-5 + MA502 + F + BAS-2 Binocular
EMZ-13 + MA502 + F + BAS-2

  • EMZ-5 + MA502 + F + BAS-2, Stereo Zoom Binocular Stand Configuration System, Magnification (7X – 45X), W.D. 93mm (3.66″)

  • EMZ-13 + MA502 + F + BAS-2, Stereo Zoom Binocular Stand Configuration System, Magnification (10X – 70X), W.D. 90mm (3.54″)

  • EMZ-8TRD + MA502 + FSC + BAS-3, Stereo Zoom Trinocular Stand Configuration System, Magnification (0.7X-0.45X), W.D. 104mm (4.1″)

  • EMZ-5H/BLACK + MA522 + F + BAS-2, Stereo Zoom Binocular Stand Configuration System, Magnification (7X – 45X), W.D. 93mm (3.66″)

  • EM-33 LED Trinocular Entry-Level 0.7X-4.5X Incident and Transmitted Zoom Stereo Microscope

  • EM-31 LED Binocular Entry-Level Dual 2x, 4x Incident and Transmitted Turret Stereo Microscope

  • EM-30 LED Binocular Entry-Level Dual 1x,3x Incident and Transmitted Turret Stereo Microscope

  • EM-32 LED Binocular Entry-Level 0.7X-4.5X Incident and Transmitted Zoom Stereo Microscope

  • EM-50L Binocular Stereo Entry-Level Industrial and Educational Microscope with 6.7X – 45X Zoom Range Magnification on a pole stand with 58 bulb array LED Ring Light (MA962) and Ring Light adapter

  • EM-51L Trinocular Stereo Entry-Level Industrial and Educational Microscope with 6.7X – 45X Zoom Range Magnification on a pole stand

  • EM-22 LED Binocular Entry-Level 1X, 2X, 3X Incident and Transmitted Turret Stereo Rechargeable Microscope

  • SKT-2BT/LED Binocular Entry-Level Microscope (1X and 3X Turret) (Limited Supply Available)

  • EM-23 LED Binocular Entry-Level 1X, 2X, 4X Incident and Transmitted Turret Stereo Rechargeable Microscope

  • EM-20 LED Binocular Entry-Level 1X, 3X Incident and Transmitted Turret Stereo Rechargeable Microscope

  • EM-21 LED Binocular Entry-Level 2X, 4X Incident and Transmitted Turret Stereo Rechargeable Microscope

  • MT5200L LED Binocular Brightfield Biological Microscope

  • MT5200H Halogen Binocular Brightfield Research/Clinical Studies Biological Microscope

  • MT5300H Halogen Trinocular Brightfield Biological Microscope

  • MT4200L Biological Compound Binocular Brightfield with Infinity Corrected U.Plan Objectives (4X, 10X, 40X, 100X Oil), LED Illumination – Magnification Range: 40X–1000X

  • MT4300L 40X-1000X Biological Compound Binocular Brightfield with Infinity Corrected U. Plan 4X, 10X, 40X, 100X, LED

  • MT4310L – 40X-400X Biological Compound Trinocular Brightfield/Phase Contrast with Infinity Corrected with 4X BF, 10X PH, 40X PH LED

  • MT4210H – 40X-400X Brightfield/Phase Contrast Biological Compound Binocular with Infinity Corrected 4X BF, 10X PH, 40X PH Halogen

  • MT4310H 40X-400X Biological Compound Ergoomic Trino Brightfield/Phase Contrast with Infinity Corrected 4X BF, 10X PH, 40X PH, Halogen

  • MT5310L LED Trinocular Brightfield/Phase Contrast Biological Microscope

  • MT4310L + HD1500TM + MA151/35/03, 40X-400X Biological Compound Trinocular Brightfield/Phase Contrast with Infinity Corrected with 4X BF, 10X PH, 40X PH LED with HD Camera Monitor (HD1500TM)

  • MT4300L + HD1500MET + MA151/35/03 LED 40X-1000X Biological Compound Binocular Brightfield with Infinity Corrected U. Plan 4X, 10X, 40X, 100X, LED with HD Camera Measurement (HD1500MET)

  • TC-5100H – Biological Inverted Binocular Brightfield Compound Microscope with Transmitted Halogen Illuminator and Infinity TC LWD Planachromat Objectives 10X and 20X

  • TC-5300L Binocular Inverted Brightfield/Phase Contrast Biological Microscope with LED illumination

  • TC-5300EL 100X, 200X Binocular Inverted Brightfield/Phase Contrast Biological Microscope with LED illumination

  • TC-5400L Binocular Inverted Brightfield/Phase Contrast Biological Microscope with LED Illumination

  • TC-5400-HD1500TM/0.3 100X, 200X Trinocular Inverted Brightfield/Phase Contrast Biological Microscope and HD Camera (HD1500TM)

  • TC-5200H – Biological Inverted Brightfield Trinocular Compound Microscope with Transmitted Halogen Illuminator and Infinity TC LWD Planachromat Objectives 10X and 20X

  • MT6200CL Binocular Epi-Fluorescence Biological Microscope with LED Light Source

  • MT6200ECL Ergonomic Tilting Binocular 10° to 50° degrees Epi-Fluorescence Biological Microscope with LED Light Source

  • MT6300CL Trinocular Epi-Fluorescence Biological Microscope with LED Light Source

  • MT6300CLM Motorized Fluorescence Microscope

  • MT6200ECW Ergonomic Tilting Binocular 10° to 50° degrees Epi-Fluorescence Biological Microscope with LED Light Source

  • MT6300CW Trinocular Epi-Fluorescence Biological Microscope with LED Light Source

  • MT6300ECL Ergonomic Tilting Trinocular 10° to 50° degrees Epi-Fluorescence Biological Microscope with LED Light Source

  • MT6200CW Binocular Epi-Fluorescence Biological Microscope with LED Light Source

  • MT6300ECW Ergonomic Tilting Binocular 10° to 50° degrees Epi-Fluorescence Biological Microscope with LED Light Source

  • TC-5500CL 100X, 200X Binocular LED/Halogen Inverted Epi-Fluorescence Biological Microscope

  • TC-5600CL Trinocular Inverted LED/Halogen Epi-Fluorescence Biological Microscope

  • TC-5500CW 100X, 200X Binocular LED/Halogen Inverted Epi-Fluorescence Biological Microscope

  • TC-5600CW Trinocular Inverted LED/Halogen Epi-Fluorescence Biological Microscope

  • MT4200L Biological Compound Binocular Brightfield with Infinity Corrected U.Plan Objectives (4X, 10X, 40X, 100X Oil), LED Illumination – Magnification Range: 40X–1000X

  • MT4300L 40X-1000X Biological Compound Binocular Brightfield with Infinity Corrected U. Plan 4X, 10X, 40X, 100X, LED

  • MT-51 Research Grade Trinocular Brightfield Upright Biological Compound Microscope with Advanced S.Plan 4X, 10X, 40X, 100X and LED Illumination

  • MT-50 Research Grade Binocular Brightfield Upright Biological Compound Microscope with Advanced S.Plan 4X, 10X, 40X, 100X and LED Illumination

  • MT-61 Trinocular Brightfield Upright Biological Compound Microscope with Infinity – Corrected Plan 4X, 10X, 40X, 100Xoil and LED Illumination

  • MT-60 Binocular Brightfield Upright Biological Compound Microscope with Infinity-Corrected Plan 4X, 10X, 40X, 100Xoil and LED Illumination

  • MT4210H – 40X-400X Brightfield/Phase Contrast Biological Compound Binocular with Infinity Corrected 4X BF, 10X PH, 40X PH Halogen

  • MT4310H 40X-400X Biological Compound Ergoomic Trino Brightfield/Phase Contrast with Infinity Corrected 4X BF, 10X PH, 40X PH, Halogen

  • MTA/MT-53 Research Grade Trinocular Brightfield Upright Biological Compound Microscope with Advanced Phase Contrast Plan Phase PH10x, PH20x, PH40x PH100x Oil and LED Illumination