Green-Only Barrier Filter
The Green-Only (GO) Barrier Filter isolates the green part of the spectrum and is for use with the Royal Blue excitation source. While our other barrier filters are long-pass filters this filter is a bandpass, transmitting from approximately 500 to 560nm. The long-pass filter has served well for most users who need to visualize green-fluorescent protein (GFP), and if you are exploring fluorescence in nature it is preferable. The primary motivation for adding the green-only filter to the line-up was for the benefit of researchers using GFP in plants such as Arabidopsis thaliana, a common research model. Plants contain chlorophyll, which has a distinctive red fluorescence that can sometimes mask the GFP emission, making it harder to see and photograph.
FLM-RB-GO Info:
The FLM-RB-GO is a stereo microscope adapter system (one illumination color) that consists of:
Designation | Excitation | Emission |
UV - Ultaviolet | 360 - 380nm | 415nm longpass |
VI - Violet | 400 - 415nm | 450nm longpass |
RB - Royal Blue | 440 - 460nm | 500nm longpass |
RB - Royal Blue | 440 - 460nm | 500-560nm bandpass |
CY - Cyan | 490 - 515nm | 550nm longpass |
GR - Green | 510 - 540nm | 600nm longpass |
Key:
Fluorescent Protein/Dye | UV | VI | RB | CY | GR | Basis |
Alexa Fluor 488 | X | W | ||||
Alexa Fluor 555 | X | s | ||||
Alexa Fluor 568 | X | s | ||||
Alexa Fluor 594 | X | s | ||||
amCyan1 | X | s | ||||
Aquamarine | X | s | ||||
Calcein | X | W | ||||
Cerulean | X | s | ||||
CFP | X | W | ||||
Citrine | X | s | ||||
Clover | X | s | ||||
Cy3 | X | s | ||||
CyOFP1 | X | s | ||||
CyPet | X | s | ||||
DAPI | X | W | ||||
Dextran-FITC | X | s | ||||
Dextran-Texas Red | X | s | ||||
Dextran-TRITC | X | s | ||||
DiI | X | s | ||||
DsRed | 2 | 1 | W | |||
E2-Orange | X | s | ||||
EBFP | X | s | ||||
EBFP2 | X | s | ||||
ECFP | X | W | ||||
EGFP | X | W | ||||
eqFP670 | X | s | ||||
EYFP | X | W | ||||
FITC | X | W | ||||
Fluorescein | X | W | ||||
FusionRed | X | W | ||||
Fluorescent Protein/Dye | UV | VI | RB | CY | GR | Basis |
GFP | X | W | ||||
Hoechst | X | W | ||||
iFP1.4 | 1 | 2 | s | |||
KO1 | X | s | ||||
LSSmOrange | X | s | ||||
Lucifer Yellow | X | W | ||||
mAmetrine | X | s | ||||
mApple | X | s | ||||
mAzamiGreen | X | s | ||||
mBanana | X | s | ||||
mBeRFP | X | s | ||||
mCardinal | X | s | ||||
mCherry1 | X | W | ||||
mCitrine | X | s | ||||
mEmerald | X | s | ||||
mGarnet2 | X | s | ||||
mHoneydew | 1 | 2 | s | |||
MiCy | X | s | ||||
mKalama1 | X | s | ||||
mKate | X | s | ||||
mKate2 | X | s | ||||
mKeima | X | s | ||||
mKO2 | X | s | ||||
mKusabira-Orange | X | s | ||||
mMidoriishi-Cyan | X | s | ||||
mNeonGreen | X | s | ||||
mNeptune | X | s | ||||
mOrange | X | s | ||||
mOrange2 | X | s | ||||
Fluorescent Protein/Dye | UV | VI | RB | CY | GR | Basis |
mPapaya1 | X | s | ||||
mPlum | X | s | ||||
mRaspberry | X | s | ||||
mRFP1 | X | s | ||||
mRFP1.2 | X | s | ||||
mRuby | X | s | ||||
mScarlet | X | s | ||||
mStable | X | s | ||||
mStrawberry | X | s | ||||
mTagBFP | X | s | ||||
mTangerine | X | s | ||||
mTurquoise2 | 1 | 2 | s | |||
mUKG | X | s | ||||
mWasabi | X | s | ||||
RFP | 2 | 1 | W | |||
Rhodamine B | X | W | ||||
Sapphire | 2 | 1 | s | |||
Superfolder GFP | X | s | ||||
SYFP2 | X | s | ||||
TagBFP | 1 | 2 | W | |||
TagCFP | X | s | ||||
TagGFP2 | X | s | ||||
TagRFP | 2 | 1 | s | |||
TagRFP-2 | 2 | 1 | s | |||
TagYFP | X | s | ||||
tdTomato | 1 | 2 | W | |||
Texas Red | X | s | ||||
TRITC | X | W | ||||
Venus | X | s | ||||
YFP | X | W | ||||
Ypet | X | s | ||||
ZsGreen | X | s |
Notes:
1 – Our Green (GR) excitation/emission set can work with mCherry but is not optimal. We know of many people who have great success and others for whom it just did not work. It is likely related to the amount of fluorescing material and the strength of the expression. It is worth trying, but you should purchase only with the understanding that you need to try it in your application and might need to return it.
The light head, barrier filter, and filter shield are interchangeable so that you can easily switch between excitation/emission light+filter combinations.
The microscope mounting adapter fits up to 67mm to work with the majority of stereo microscopes.
Once you are set up for one excitation/ emission wavelength combination, additional combinations can be added by purchasing a kit that consists of a light head, barrier filter, and viewing shield. These three elements can be removed and replaced in seconds, and color coding ensures that you are using the right combination. The barrier filter clicks on to the ring adapter magnetically, so it is easy to remove it to switch back to white light viewing.
Designation | Excitation | Emission |
UV - Ultaviolet | 360 - 380nm | 415nm longpass |
VI - Violet | 400 - 415nm | 450nm longpass |
RB - Royal Blue | 440 - 460nm | 500nm longpass |
RB - Royal Blue | 440 - 460nm | 500-560nm bandpass |
CY - Cyan | 490 - 515nm | 550nm longpass |
GR - Green | 510 - 540nm | 600nm longpass |
This Stereo Microscope Fluorescence Adapter system (SFA) is a complete solution for adapting existing stereo microscopes to visualize fluorescence. It includes everything you need for adapting (not converting) just about any stereo microscope, including light sources and matching barrier filters. It is a modular system, so you can purchase the wavelength combinations that you need now and add more at any time. We now offer 6 excitation/emission combinations and add more as we can.
You can utilize existing stereo microscopes for fluorescence at a very reasonable cost. This opens up a range of possibilities
Add-on excitation/emission set consists of