Polarizer and Wave Plate Set Up
1. Rotate the
full wave plate out of the light path as
shown below:
2. Turn on the illuminator.
3. Slide the Analyzer into the optical path.
4. Look into the microscope. The polarizer
has already been positioned so that the
image is at extinction, so the field of
view should be black.
5. Tighten the
locking screw to secure your setting.
Identifying
the Crystals - Gout
The following
procedure is for identification of gout (for
pseudo-gout, see the next section). To
ensure the accuracy of the test, a slide of
known monosodium urate crystals should be
used for reference.
1. Swing the
full wave plate out of the light path.
2. Place the
slide onto the stage and bring the crystals
into sharp focus. The needle-shaped crystals
in every direction should appear white.
3. Swing in the
full wave plate with the lever in the leftmost
position. Crystals pointing in the
North-South direction should appear yellow;
crystals in the East-West direction should
appear blue. (see Figure 1)
Figure 1
4. Move the
lever to the extreme right position and the
reverse should occur: North-South crystals
are now blue and East-West crystals are now
yellow (see Figure 2). This confirms the
presence of gout.
Figure 2
Identifying the Crystals - Pseudo-gout
The following
procedure is for identification of
pseudo-gout. To ensure the accuracy of the
test, a slide of known calcium pyrophosphate
dehydrate crystals should be used for
reference.
1. Swing the
full wave plate out of the light path.
2. Place the
slide onto the stage and bring the crystals
into sharp focus. The needle-shaped crystals
in every direction should appear white.
3. Swing in the
full wave plate with the lever in the leftmost
position. Crystals pointing in the
North-South direction should appear blue.
Crystals in the East-West direction should
appear yellow (see Figure 3).
Figure 3
4. Move the
lever to the extreme right position and the
reverse should occur: North-South crystals
are now yellow, and East-West crystals are
now blue (see Figure 4). this confirms the
presence of pseudo-gout.
Figure 4
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