TC-5300L Binocular Inverted Brightfield/Phase Contrast Biological Microscope with LED illumination

Our latest inverted microscope line is the cost-effective TC-5000 Series Inverted Biological Microscopes. The TC Series makes routine examining of tissue cultures, microbes and other live specimens in petri dishes and culture bottles fast and easy while delivering an excellent performance-to-cost ratio. The TC Series is available in brightfield, phase contrast and epi-fluorescence models. The TC5000 Series Microscopes have an integrated front mounted camera port with adapters available for 35mm, CCD/CMOS and other cameras.

The TC-5300 and TC-5400 Series are suitable for viewing colorless and transparent specimens and live cells. The TC-5300 and TC-5400 Series are contrast-enhancing optical tools that can be utilized in producing high-contrast images of transparent specimens such as living cells, microorganisms, thin tissue slices, lithographic patterns, and sub-cellular particles (such as nuclei and other organelles). In effect, the phase contrast technique employs an optical mechanism to translate minute variations in phase into corresponding changes in amplitude. One of the major advantages of phase contrast microscopy is that living cells can be examined in their natural state without being killed, fixed, and stained. As a result, the dynamics of ongoing biological processes in live cells can be observed and recorded with sharp view of the specimen.

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Description

The TC-5000 Brightfield/Phase Contrast Series features include:

  • Seidentopf type binocular head inclined at 30° or trinocular at 45°
  • Equipped with LED transmitted lamp illuminator and Mercury Lamp Incident illuminator
  • N.A. 0.30 condenser
  • 180mm(X) X 245mm(Y) stage with replaceable glass insert with 45mm opening
  • Exchangeable observation tubes
  • Integrated Automatic Voltage Sensing Power Supply with localized power cord
  • Compact wedge shaped cast aluminum alloy frame for exceptional stability and a small footprint
  • Chemical resistant baked enamel finish
  • Flexible and modular design for system expansion
  • Transparent stage plate for easy objective identification
  • Low profile coaxial and fine focus controls with tension adjustment
  • Precentered LED Lamp
  • Front mounted CCD, analog or SLR cameras
  • Integrated Automatic Voltage Sensing Power Supply with localized power cord
  • Optional mechanical stage with specimen holders

Brightfield:
The simplest of all the optical microscopy illumination techniques. Sample illumination is transmitted (i.e., illuminated from below and observed from above) white light and contrast in the sample is caused by absorbance of some of the transmitted light in dense areas of the sample. Brightfield microscopy is the simplest of a range of techniques used for illumination of samples in light microscopes and its simplicity makes it a popular technique. The typical appearance of a brightfield microscopy image is a dark sample on a bright background.

Phase Contrast:
An optical microscopy technique that converts phase shifts in light passing through a transparent specimen to brightness changes in the image. Phase shifts themselves are invisible, but become visible when shown as brightness variations. When light waves travel through a medium other than vacuum, interaction with the medium causes the wave amplitude and phase to change in a manner dependent on properties of the medium. Changes in amplitude (brightness) arise from the scattering and absorption of light, which is often wavelength-dependent and may give rise to colors. Photographic equipment and the human eye are only sensitive to amplitude variations. Without special arrangements, phase changes are therefore invisible.

The TC-5300 & TC-5400 Series are suitable for viewing colorless and transparent specimens and live cells. The TC-5300 & TC-5400 Series is a contrast-enhancing optical technique that can be utilized to produce high-contrast images of transparent specimens such as living cells, microorganisms, thin tissue slices, lithographic patterns, and sub-cellular particles (such as nuclei and other organelles). In effect, the phase contrast technique employs an optical mechanism to translate minute variations in phase into corresponding changes in amplitude, which can be visualized as differences in image contrast. One of the major advantages of phase contrast microscopy is that living cells can be examined in their natural state without being killed, fixed, and stained. As a result, the dynamics of ongoing biological processes in live cells can be observed and recorded in high contrast with sharp clarity of minute specimen detail.

Additional information

Weight 12.0000 kg
Components & Accessories

Included:    • TC-5300: Binocular Inverted Phase Contrast/Brightfield Compound Microscope, ∞ Infinity Corrected Optical System F-200MM with LED illumination with quintuple nosepiece and auto voltage sensing power supply    • MA814: Siedentopf type binocular, inclined at 30°, Interpupillary adjustment 53mm to 75mm graduated diopter on left eyetube (30mm I.D. eyetubes)    • MA817 (x 2): SWH10X Widefield high eyepoint eyepiece, Field No. 22, tube O.D. 30.0 mm    • MA826: TC Long Working Distance LWD Planachromat Phase 10X objective ∞/1.0     • MA827: TC Long Working Distance LWD Planachromat Phase 20X objective ∞/1.0    • MA853: TC Extra Long Working Distance  ELWD Condenser, N.A. 0.30, W.D. 73.0mm    • MA861: Green interference filter in 51mm mount (546mm)    • MA863: Cobalt clear filter in mount in 51mm mount    • STAGE: Plain stage with 180mm(X) x 245mm(Y) plain stage with replaceable glass insert with 45mm opening    • MA878: Glass Stage plate insert for plain stage (Round-109mm diameter, 1.5mm thick with 44.3mm central opening)    • MA855: Phase slider    • MA458/05: Centering telescope (O.D. = 30.0)    • MA809/10: Removable AC Electric cord with plug for 115V models     • MA326: Bulb 6V 30W Halogen (Factory Installed)    • MA327: Fuse 3 Amp (Factory Installed) OPTIONAL COMPONENTS & ACCESSORIES BELOW Viewing Heads:      MA816/10: Siedentopf type trinocular head, inclined at 30°, I.P. adjustment 53mm to 75mm graduated diopter on left eyetube (30mm I.D. eyetubes) (Included with TC-5400) Includes 80/20 beamsplitter (100% to eyetubes or 80% to photo 20% to eyetube)     MA957/15: Ergonomic binocular head, inclination adjustable vertically from 10° to 50° (Included with TC-5100E)     MA1034: Trinocular Siedentopf head, erect image, inclined at 30°, I.P. adjustment 53mm to 75mm, graduated diopter on left eyetube (30mm I.D. eyetubes) Includes 0/100 beamsplitter (100% to eyetubes or 100% to phototube) Eyepieces:     MA818: SWH 15X Widefield high eyepoint eyepiece, Field No. 16.0, tube O.D. 30.0 mm (each) (19mm reticle mount)     MA819: SWH 20X Widefield high eyepoint eyepiece, Field No. 12.0, tube O.D. 30.0 mm (each) (19mm reticle mount)     MA859: SWH 10X-F Focusing eyepiece with reticle mount (without reticle), tube O.D. 30.0 mm (each) (25mm reticle mount) Phase Objectives:      MA825: TC Planachromat Phase 4X objective ∞/1.0 N.A. 0.13, F.L. 50.0, W.D. 17.3     MA828: TC Planachromat Phase 40X objective ∞/1.0 N.A. 0.65, F.L. 5.0, W.D. 2.8 Condensers:      MA854: TC Condenser, N.A. 0.55, W.D. 20.5mm (For use with MA823 40X objective) Filters:     MA856: LB100 Blue clear filter in 51mm mount     MA857: G533 Green clear filter in 51mm mount     MA858: ND25 Neutral density filter in 51mm mount
Miscellaneous:     MA809/20: Removable AC Electric cord with plug for 220V models (included in all 220V models) CAMERA ADAPTERS FOR “C” MOUNT TYPE CAMERAS     MA877: “C” mount type camera adapter for CCD/CMOS camera (No internal lens) (Magnification factor of 0.66X from TC5000 Series front camera port) CAMERA ADAPTERS FOR DIGITAL STILL CAMERAS     MA868: Digital camera adapter for non “C” mount type cameras (52mm O.D. thread) For TC Series front camera port only: (Requires additional DCA adapter ring for your specific model of camera which are listed on the last two pages of the TC5000 section) CAMERA ADAPTERS FOR 35mm TYPE CAMERAS     MA869: 35mm SLR camera attachment Magnification at film plane is 2.23X of objective in use
Stages (Fixed 245mm x 180mm, plain stage is included with stand):     MA878: Replacement glass stage plate insert for plain stage – TC5000 Series (Round – 109mm diameter, 1.5mm thick with 44.3mm opening) (Fits plain stage only not for use with MA380/05)     MA380/05: Attachable mechanical stage, movement 112mm(X) x 72mm(Y) with test plate holder and drop-down coaxial X-Y controls. COMPONENTS OFTEN PAIRED WITH THE MA380/05 MECHANICAL STAGE Specimen Holders for use with MA380/05 Attachable Mechanical Stage (Rectangular Plate 129.74mm x 89.78 mm x 3mm):     MA382: Slide glass holder, 1”x 3”      MA383: Chamber holder (For 55mm x 80mm chamber)     MA384: Petri dish holder 35mm diameter     MA385: Petri dish holder 55mm diameter     MA387: Petri dish holder 65mm diameter     MA864: Terasaki plate holder (For 56mm x 87mm Terasaki plate)     MA378: Hemacytometer holder Stage Micrometers:     MA285: Objective micrometer, 0.01mm ruling     MA286: 0.04” divided into 40 units, 0.001     MA285: 1mm divided into 100 parts Eyepiece Micrometers:     MA523: Cross-line reticule, 25mm diameter     MA506: Eyepiece micrometer, 10mm divided into 100 units, 25mm diameter     MA509: Eyepiece micrometer 5mm divided into 100 units, 25mm diameter     MA524: 10mm square divided into 100 parts, 1.0mm square, 25mm diameter     MA542: Cross-line with 0.1mm graduations, 21mm diameter

Details

Model

Head

Illumination

Eyepieces

Objectives

Stage

Condenser

TC-5100 Brightfield

Binocular

Koehler w/o iris 6V 30W halogen

SWH10x FN22

Quintuple Nosepiece w/LWD 10X &LWD 20X

Plain Fixed Stage180mm X 247mm

TC Condenser WD 73mmNA 0.30orTC Condenser2 WD 20.5mmNA 0.55

TC-5100E Brightfield

Ergonomic Binocular

TC-5200 Brightfield

Trinocular

TC-5300 and TC-5300L Phase Contrast

Binocular

Koehler w/o iriswith phase slider 6V 30W halogenKoehler 6V 30W halogen

TC-5300E or TC-5300EL Phase Contrast

Ergonomic Binocular

TC-5400 or TC5400L Phase Contrast

Trinocular

TC-5500 Epi-Fluorescent

Binocular
100W Mercury + Koehler w/o iris   6V 30W halogen

Quintuple Nosepiece TC Planachromat F10X, F20X

TC Condenser WD 73mm NA 0.30 or TC Condenser2 WD 20.5mm NA 0.55

TC-5600 Epi-Fluorescent

Trinocular

Downloads

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Double click to read at full screen Download Manual (PDF)

Specifications

Model

Head

Illumination

Eyepieces

Objectives

Stage

Condenser

TC-5100 Brightfield

Binocular

Koehler w/o iris 6V 30W halogen

SWH10x FN22

Quintuple Nosepiece w/LWD 10X &LWD 20X

Plain Fixed Stage180mm X 247mm

TC Condenser WD 73mmNA 0.30orTC Condenser2 WD 20.5mmNA 0.55

TC-5100E Brightfield

Ergonomic Binocular

TC-5200 Brightfield

Trinocular

TC-5300 and TC-5300L Phase Contrast

Binocular

Koehler w/o iriswith phase slider 6V 30W halogenKoehler 6V 30W halogen

TC-5300E or TC-5300EL Phase Contrast

Ergonomic Binocular

TC-5400 or TC5400L Phase Contrast

Trinocular

TC-5500 Epi-Fluorescent

Binocular
100W Mercury + Koehler w/o iris   6V 30W halogen

Quintuple Nosepiece TC Planachromat F10X, F20X

TC Condenser WD 73mm NA 0.30 or TC Condenser2 WD 20.5mm NA 0.55

TC-5600 Epi-Fluorescent

Trinocular

Includes:    • TC-5300L: Binocular Inverted Phase Contrast/Brightfield Compound Microscope, ∞ Infinity Corrected Optical System F-200MM quintuple nosepiece and auto voltage sensing power supply    • MA814: Siedentopf type binocular, inclined at 30°, Interpupillary adjustment 53mm to 75mm graduated diopter on left eyetube (30mm I.D. eyetubes)    • MA817 (x 2): SWH10X Widefield high eyepoint eyepiece, Field No. 22, tube O.D. 30.0 mm    • MA826: TC Long Working Distance LWD Planachromat Phase 10X objective ∞/1.0    • MA827: TC Long Working Distance LWD Planachromat Phase 20X objective ∞/1.0    • MA853: TC Extra Long Working Distance  ELWD Condenser, N.A. 0.30, W.D. 73.0mm    • MA861: Green interference filter in 51mm mount (546mm)    • MA863: Cobalt clear filter in mount in 51mm mount    • STAGE: Plain stage with 180mm(X) x 245mm(Y) plain stage with replaceable glass insert with 45mm opening    • MA878: Glass Stage plate insert for plain stage (Round-109mm diameter, 1.5mm thick with 44.3mm central opening)    • MA855: Phase slider    • MA458/05: Centering telescope (O.D. = 30.0)    • MA809/10: Removable AC Electric cord with plug for 115V models    • MA326: Bulb 6V 30W Halogen (Factory Installed)    • MA327: Fuse 3 Amp (Factory Installed)    • Manual: TC-5000 Meiji Techno Instruction Manual    • Warranty: Limited Lifetime Warranty Card  Product Information: Manufactured: 100% MADE IN JAPANWarranty: LIMITED LIFETIME WARRANTY Customer Support: 1-(800) 832-0060 (U.S.A Based) Package Information: Weight: ~35 lbs (15.8 kg)Box Size: 19 x 15 x 25 inches Binocular head type: 485 mm(D) x 411 mm(H) x 220 mm(W) (8.0kg)( 29.0 lbs)Trinocular head type: 485 mm(D) x 440 mm(H) x 2210 mm(W) (8.5Kg)(31.0 lbs)Binocular Ergonomic head type: 485 mm(D) x 440mm(H) x 2210mm(W) (8.5Kg)(31.0 lbs)

VIEWING HEADS Binocular models, the TC5100, TC5300 and TC5500, incorporate the MA814 Siedentopf-type binocular head while the trinocular models, the TC5200, TC5400 and TC5600 have the MA816/10 Siedentopf-type trinocular head. The MA816/10 uses an 80/20 beamsplitter that can be engaged for photo-microscopy. (100% to eyetubes or 80% to phototube and 20% to the eyetube.) Each microscope head has the eyetubes inclined at 30 degrees with the left eyetube having graduated diopter settings. The interpupillary distance on the viewing heads is adjustable between 53mm – 75mm. 
EYEPIECES 10X Super Widefield High Eyepoint eyepieces are standard and 15X and 20X eyepieces are also available as an option. A Super Widefield High Eyepoint 10X focusable eyepiece that accepts standard 25mm reticules is also available.
OBJECTIVE CHANGER Quintuple nosepiece provides effortless objective changes with smooth-operating, ball bearing mounted.
OBJECTIVES Meiji Techno America offers an assortment of Planachromat Infinity Corrected (ICOS) objectives for Brightfield, Darkfield and Phase Contrast observation modes.
STAGE The optional attachable mechanical stage measures 112mm(X) x 72mm(Y). It has ergonomically positioned right-handed coaxial drop down controls. It also accepts the following optional accessory plates: 35mm, 55mm, 65mm, Petri Dish Holders, 96-well Terasaki Plate Holder, 1" X 3" glass slide holder, Hemacytometer holder.Slide, chamber, and petri dish holding plates of various sizes are available separately.  
CONDENSER The standard condenser has a working distance of 73mm.
ILLUMINATION A powerful LED bulb with pre-centered socket provides enhanced image quality and brightness for the observation of specimens.
PHOTOMICROGRAPHY Meiji Techno offers a broad range of digital video cameras which offers excellent sensitivity, high resolution and a wide dynamic range for the most demanding brightfield and darkfield microscopy applications including clinical pathology and cytology, life sciences and geology. Some models has included software, that can preview and capture still shots and live videos to a PC or Mac with USB port or HDMI connectivity.