TC-5400L-HD1000-LITE-M/0.3 100X, 200X Binocular Inverted Brightfield/Phase Contrast Biological Microscope with LED Illumination and HD Camera

Includes:
   • TC-5400L: Trinocular Inverted Phase Contrast/Brightfield Compound microscope, with ∞Infinity Corrected Optical System, F-200MM with LED Koehler illumination with quintuple nosepiece and auto voltage sensing power supply
   • HD1000-LITE-M: The HD1000-LITE is a HDMI /USB2.0 camera technology from Meiji Techno! It is the most economical HDMI & USB 2.0 camera in the market. You can easily operate the HD1000-LITE camera by directly connecting it to a monitor with a HDMI port without a PC and directly save images into an on-board SD-4G card on the camera, or connect the camera directly to your PC as it has a standard USB 2.0 interface to utilize the full annotation/software function
   • MA151/35/03: C-mount adapter with 0.3X lens
   • MA816/10: Siedentopf type trinocular head, inclined at 30°, Interpupillary adjustment 53mm to 75mm graduated diopter on left eyetube (30mm I.D. eyetubes)
 
   • MA817 (x 2): SWH10X Widefield high eyepoint eyepiece, Field No. 22, tube O.D. 30.0 mm

   • MA826: TC Long Working Distance LWD Planachromat Phase 10X objective ∞/1.0
   • MA827: TC Long Working Distance LWD Planachromat Phase 20X objective ∞/1.0

   • MA853: TC Extra Long Working Distance  ELWD Condenser, N.A. 0.30, W.D. 73.0mm
   • MA861: Green interference filter in 51mm mount (546mm)
   • MA863: Cobalt clear filter in mount in 51mm mount
   • STAGE: Plain stage with 180mm(X) x 245mm(Y) plain stage with replaceable glass insert with 45mm opening

   • MA878: Glass Stage plate insert for plain stage (Round-109mm diameter, 1.5mm thick with 44.3mm central opening)
   • MA855: Phase slider

   • MA458/05: Centering telescope (O.D. = 30.0)
   • MA809/10: Removable AC Electric cord with plug for 115V models
   • MA326: Bulb LED (Factory Installed)
   • MA327: Fuse 3 Amp (Factory Installed)
   • Manual: TC-5000 Meiji Techno Instruction Manual
   • Warranty: Limited Lifetime Warranty Card 

VIEWING HEADS

   • HD1000-LITE - Camera HDMI 1/3 “ CMOS CHIP
   • HDMI to HDMI Cable 3 foot long cable
   • USB 2.0 to USB2.0 cable
   • Full SD CARD-H4SD card(4G)
   • Power Supply 12V AC./DC adapter
   • USB 2.0 Mouse
   • Measurement Software with Annotation Feature Installation Disc
   • 11.6" Monitor

Package Information:
   • 5lbs
   • 12 x 10 x 4 inches

NEW: BUILT-IN MOUSE CONTROL CAMERA
The significant innovation of the HD1000-LITE is making the software implant inside the camera. This forward thinking feature frees users from cumbersome computers and annoying buttons. You can control the camera by only a mouse directly.

Videos of the camera in action with our microscopes:
Brief overview with the compare feature
With Darkfield microscopy.

PERFECT COLOR REPRODUCTION
Meiji Techno's HD technology offers true-to-life reproduction on image color. What you see on the monitor is what you get from the microscope eyepiece. No compressed date transmission, resulting in scientific-grade images.

HDMI+USB 2.0+SD OUTPUT
Multiple output options are available for this powerful HDMI camera. HDMI 1080P, USB 2.0, or SD memory card can be used individually or all three SIMULTANEOUSLY.

DUAL FPGA PROCESSORS
With an innovative Dual FPGA processor architecture design, FPGA1 for image quality processing and FPGA2 for image output control, you get a high-speed preview without sacrificing image quality. It is the most powerful camera you deserve to have.

SMART PRESENTATION
Camera parameters such as exposure time, white balance, and gain are designed by default to operate automatically. Automatic optimum configuration is expected to be done in 0.015 seconds, and you'll find that the camera is easily managed from the start.

ADVANCED PARAMETER SETTING
For users with specific requirements, the camera is designed with parameter setting buttons that enable manual adjustment of white balance, gain, saturation, Gamma and screen freeze.

PC TO TV
Images are displayable on PCs and HDMI-installed TVs. All parameters can be controlled by the computer to adjust them to actual situations. This is advantageous in teaching and meeting applications.

On-board Video Saving
The SD card can save not only still images, but also dynamic images in 720P color definition, such that motion trails are recorded in fast speed and intact form.

LOW LIGHT SENSITIVITY
Thanks to its highly sensitive sensor, the HD1000-LITE is capable of capturing low-light images rapidly. In darkfield imaging and even fluorescence, it will automatically increase the sensitivity, presenting low light images with clarity in high-speed preview mode.

Frequently Asked Questions

Q: Start the software and get the camera recognized, but the preview is all black.
A: Check whether the anti-dust cap on the C-mount is removed. If already removed and attach the camera to the microscop, check whether the lighting path toggle switch to the camera. Then try to extend the exposure time and gain to get brighter images.

Q: The imaging scene from the eyepiece is in focus but the live images from the camera are still out of focus.
A: This is because the camera and the eyepiece are in different focal planes. Can consider to use adjustable adapter to connect camera to the microscope trinocular, and the adjust the adapter to make the live image in focus.

Q: There are some shadows on the image and the shadows always stay in the same position even when the sample is moved.
A: Possible reasons:
1. The anti-dust film on the camera C-mount is not removed. Remove the anti-dust film
2. Check to see if there is any dirt on the objective. Clean the objective.
3. Some dust on the surface of the camera IR filter. Clean the camera optical port.

Q: Camera frame rate is extremely slow.
A: Possible reasons:
1. Preview resolution is too high. Try lowering the resolution and check the change of the frame rate.
2. Exposure time is too long. If need exposure time longer than 200ms, try to use higher gain to get brighter images.
3. Computer issue. Computer is very old or the motherboard chipset is Intel 8 Series/C220 series.

Q: Start the software and get the error message: can not recognize the camera or No camera.
A: Possible reasons:
1. The camera USB cable is not well connected. Reconnect the camera again.
2. Check whether the camera is connect to the USB port on the front of the mainframe. If so, then connect it to the USB port on the back of the mainframe. The USB ports on the front of the mainframe are the extended ports from the motherboard which can not provide stable voltage and current for cameras.
3. The camera itself needs extra power supply but does not switch on the power. TrueChrome is required to switch on power, attach to the PC and then restart the software. TCC-6.1ICE is required to plug in power to be recognized by PC.
4. The camera driver is not installed or failed to install. Go to the "Device Manager" > "Imaging Devices" and check whether the camera is listed WITH YELLOW FLAG. Then re-install the driver again. If still fail to install to driver, please contact camera technical support for help.

Q: Image Field of View (FOV) is much smaller than the scene can be seen from the eyepieces.
A: Possible reasons:
The camera sensor size is not 1 inch. It needs reduce lens to get bigger FOV.
Low resolution is used to preview. Set full resolutionion.
The reduce lens does not match the camera sensor size.

Q: When push “Capture” button, sometimes the saved images are quite blurry.
A: It could be because some slight vibration happens on the camera during capturing the images.

Q: Security shield on ISCapture shortcut.
A: Some anti-virus or firewall softwaree installed in the PCs will identify the DLL files from ISCapture as virus and automatically isolate or delete the ISCapture software files. If have the warning message to delete or isolate the DLL files during the ISCapture installation, please ignore the warning message or set the ISCapture as trusted software. Otherwise, the ISCapture can not be used normally.

Q: Newer version software con not recognize the old cameras.
A: Currently, all the newer version software ISCapture is compatible with the previous version cameras EXCEPT TCA Series.

Q: After doing the measurement on the live images, the captured image is without any measurement data.
A: To save the live measurement on captured images, check the “Combined Measurements” checkbox in the File Save tab and then capture images. While doing the still image measurements, only click the Save or Save As on the right hand side shortcut to save the data on the images.

Q: The live images and the captures images color is quite different from the one we can see from the eyepieces.
A: Because of the different lighting source, White Balance is needed to correct the image color.

Q: How to do quick White Balance?
A: White Balance while attached to camera to stereo microscope: Use a white paper to replace the sample. Click on “White Balance” to correct the image color > Remove the white paper and put back the sample.
White Balance while attach the camera to the biological microscope: Move the sample slide to the blank area. Click on “White Balance” and move back the sample.

Q: After install ISCapture and firstly start the software, but get the error “The program can’t start because MSVCP100.dll is missing from your computer. Try reinstalling the program to fix this problem.”

A; This is because the operating system lack of some Visual C++ 32bit library files which the ISCapture needs to call them. Without these files, the software can not be started normally. Install the patch VCredist_x86.exe (can be found in the CD that comes with the camera or go to http://www.microsoft.com/en-us/download/details.aspx?id=5555

Q: Start the ISCapture but get the error “Unable to execute file C:\Program Files\ISCapture\ISCapture.exe CreateProcess failed; code 740.

A: Completely remove the ISC Capture, reboot the PC and then right click on ISCapture installer (ISCapture Setup.exe) and select “Run as Administrator” to install the ISCapture. When you first start the software right click on the ISC icon and select “Run as Administrator” to start it.

Q:When start ISCapture, get error message “Cannot find the USB Key!”

A: The software is encrypted. Specific USB key is needed to attach to the computer port first.

Q:After capturing several images, it takes more and more time to save a frame image. Sometimes it is even up to more than 30 seconds, and then finally the software hang or crash.
A: If the image save directory is the network driver, during uploading the files to network driver, it will significantly increase the COP usage. When the CUP usage is up to 100%, the software will hang or crash. Recommend to change the file save directory to the computer hard disk.

Q: Whether can share the calibration files on different PCs?
A: If using the ISCapture earlier version than V4.0: Go to the folder “Application Data/ISCapture” in system disk, find the file “calibrationTable.ini” and copy it to another PC under the same directory. (The “Application Data” folder is a hidden folder, you might need to make the system invisible visible first.)
If it is ISCapture V4.0 or later, go to the Parameter tab and click “Backup” to backup the parameter settings and copy them to another PC to restore them.

Q:After install the ISCapture and every time I run the software i get the error:

A: This is because some program in the user’s PC is using the library file ‘libiomp5md.dll’which will conflict with the ISCapture. So far as we know, the software GoPro Studio will conflict with ISCapture. To solve this issue, please try to remove the libiomp5md.dll from the C:\Windows\System32 folder while using the ISCapture.

Q: Shortcut keys for ISCapture to capture still images and videos:
A: With ISCapture V3.6.6 or later, running the ISCapture first, Press F9 key on the keyboard to capture images. Press F10 to start the video recording. If in manual recording mode, press F11 to stop the recording.

Q: On preview images, lots of horizontal lines appear.

A: This is because of the lighting frequency. Extend exposure time or turn off the filament lamp or fluorescent lamp in the environment to solve this issue.

Q:Recording issue when connected to the HD1500T/HD1000 Series to the monitor and recorded video directly to the SD card? If so, do you know what the capacity of the SD card the user used during recording the videos?
A:When recording videos to the SD card, the camera only supports the SD card with format SDHC. It can not work with the SD card format SDXC. And max. capacity of the SDHC format SD card is 32GB which means the SD card with capacity >= 64GB, is format SDXC.
If already use SDHC format SD card, please help to confirm the information below:
1). Whether the customer used the SD card came with the HD1500T/HD1000 camera?
2). If not, what exactly the model of the SD card the customer used? Maybe some picture of the SD card.

If got the issue while connect the camera to the computer, I need to confirm below information:
1). The software always stop recording AUTOMATICALLY after 5-10 minutes?
2). Whether the software or the computer freeze if MANUALLY stop the recording?

Product Information: 
   • Manufactured: 100% MADE IN JAPAN
   • Warranty: LIMITED LIFETIME WARRANTY 
   • Customer Support: 1-(800) 832-0060 (U.S.A Based)

TC-5400L Package Information: 
   • Weight: ~35 lbs (15.8 kg)
   • Box Size: 19 x 15 x 25 inches 
   • Trinocular head type: 485 mm(D) x 440 mm(H) x 2210 mm(W) (8.5Kg)(31.0 lbs)

HD1000-LITE-M Package Information:
   • 5lbs
   • 12 x 10 x 4 inches

The TC-5000 Brightfield/Phase Contrast Series features include:

• Seidentopf type binocular head inclined at 30° or trinocular at 45°
• Equipped with 6V, 30W Halogen lamp illuminator
• N.A. 0.30 condenser
• 180mm(X) X 245mm(Y) stage with replaceable glass insert with 45mm opening
• Exchangeable observation tubes
• Integrated Automatic Voltage Sensing Power Supply with localized power cord
• Compact wedge shaped cast aluminum alloy frame for exceptional stability and a small footprint
• Chemical resistant baked enamel finish
• Flexible and modular design for system expansion
• Transparent stage plate for easy objective identification
• Low profile coaxial and fine focus controls with tension adjustment
• Precentered LED bulb
• Front mounted CCD, analog or SLR cameras
• Integrated Automatic Voltage Sensing Power Supply with localized power cord
• Optional mechanical stage with specimen holders

Brightfield:
The simplest of all the optical microscopy illumination techniques. Sample illumination is transmitted (i.e., illuminated from below and observed from above) white light and contrast in the sample is caused by absorbance of some of the transmitted light in dense areas of the sample. Brightfield microscopy is the simplest of a range of techniques used for illumination of samples in light microscopes and its simplicity makes it a popular technique. The typical appearance of a brightfield microscopy image is a dark sample on a bright background.

Phase Contrast:
An optical microscopy technique that converts phase shifts in light passing through a transparent specimen to brightness changes in the image. Phase shifts themselves are invisible, but become visible when shown as brightness variations. When light waves travel through a medium other than vacuum, interaction with the medium causes the wave amplitude and phase to change in a manner dependent on properties of the medium. Changes in amplitude (brightness) arise from the scattering and absorption of light, which is often wavelength-dependent and may give rise to colors. Photographic equipment and the human eye are only sensitive to amplitude variations. Without special arrangements, phase changes are therefore invisible.

The TC-5300 & TC-5400 Series are suitable for viewing colorless and transparent specimens and live cells. The TC-5300 & TC-5400 Series is a contrast-enhancing optical technique that can be utilized to produce high-contrast images of transparent specimens such as living cells, microorganisms, thin tissue slices, lithographic patterns, and sub-cellular particles (such as nuclei and other organelles). In effect, the phase contrast technique employs an optical mechanism to translate minute variations in phase into corresponding changes in amplitude, which can be visualized as differences in image contrast. One of the major advantages of phase contrast microscopy is that living cells can be examined in their natural state without being killed, fixed, and stained. As a result, the dynamics of ongoing biological processes in live cells can be observed and recorded in high contrast with sharp clarity of minute specimen detail.

Included:
   • TC-5400: Trinocular Inverted Phase Contrast/Brightfield Compound microscope, with ∞Infinity Corrected Optical System, F-200MM with LED illumination with quintuple nosepiece and auto voltage sensing power supply
   • MA816/10: Siedentopf type trinocular head, inclined at 30°, Interpupillary adjustment 53mm to 75mm graduated diopter on left eyetube (30mm I.D. eyetubes)
   • MA817 (x 2): SWH10X Widefield high eyepoint eyepiece, Field No. 22, tube O.D. 30.0 mm
   • MA826: TC Long Working Distance LWD Planachromat Phase 10X objective ∞/1.0 
   • MA827: TC Long Working Distance LWD Planachromat Phase 20X objective ∞/1.0
   • MA853: TC Extra Long Working Distance  ELWD Condenser, N.A. 0.30, W.D. 73.0mm
   • MA861: Green interference filter in 51mm mount (546mm)
   • MA863: Cobalt clear filter in mount in 51mm mount
   • STAGE: Plain stage with 180mm(X) x 245mm(Y) plain stage with replaceable glass insert with 45mm opening
   • MA878: Glass Stage plate insert for plain stage (Round-109mm diameter, 1.5mm thick with 44.3mm central opening)
   • MA855: Phase slider
   • MA458/05: Centering telescope (O.D. = 30.0)
   • MA809/10: Removable AC Electric cord with plug for 115V models 
   • MA326: Bulb 6V 30W Halogen (Factory Installed)
   • MA327: Fuse 3 Amp (Factory Installed)
   • HD1000-LITE-M: The HD1000-LITE is a HDMI /USB2.0 camera technology from Meiji Techno! It is the most economical HDMI & USB 2.0 camera in the market. You can easily operate the HD1000-LITE camera by directly connecting it to a monitor with a HDMI port without a PC and directly save images into an on-board SD-4G card on the camera, or connect the camera directly to your PC as it has a standard USB 2.0 interface to utilize the full annotation/software function
   • MA151/35/03: C-mount adapter with 0.3X lens

OPTIONAL COMPONENTS & ACCESSORIES BELOW

Viewing Heads:
   • MA814: Siedentopf type binocular, inclined at 30°, Interpupillary adjustment 53mm to 75mm graduated diopter on left eyetube (30mm I.D. eyetubes)
   • MA957/15: Ergonomic binocular head, inclination adjustable vertically from 10° to 50° (Included with TC-5100E)
   • MA1034: Trinocular Siedentopf head, erect image, inclined at 30°, I.P. adjustment 53mm to 75mm, graduated diopter on left eyetube (30mm I.D. eyetubes) Includes 0/100 beamsplitter (100% to eyetubes or 100% to phototube)

Eyepieces:
   • MA818: SWH 15X Widefield high eyepoint eyepiece, Field No. 16.0, tube O.D. 30.0 mm (each) (19mm reticle mount)
   • MA819: SWH 20X Widefield high eyepoint eyepiece, Field No. 12.0, tube O.D. 30.0 mm (each) (19mm reticle mount)
   • MA859: SWH 10X-F Focusing eyepiece with reticle mount (without reticle), tube O.D. 30.0 mm (each) (25mm reticle mount)

Phase Objectives:
   • MA825: TC Planachromat Phase 4X objective ∞/1.0 N.A. 0.13, F.L. 50.0, W.D. 17.3
   • MA828: TC Planachromat Phase 40X objective ∞/1.0 N.A. 0.65, F.L. 5.0, W.D. 2.8

Condensers:
   • MA854: TC Condenser, N.A. 0.55, W.D. 20.5mm (For use with MA823 40X objective)

Filters:
   • MA856: LB100 Blue clear filter in 51mm mount
   • MA857: G533 Green clear filter in 51mm mount
   • MA858: ND25 Neutral density filter in 51mm mount

Miscellaneous:
   • MA809/20: Removable AC Electric cord with plug for 220V models (included in all 220V models) CAMERA ADAPTERS FOR “C” MOUNT TYPE CAMERAS
   • MA877: “C” mount type camera adapter for CCD/CMOS camera (No internal lens) (Magnification factor of 0.66X from TC5000 Series front camera port)
CAMERA ADAPTERS FOR DIGITAL STILL CAMERAS
   • MA868: Digital camera adapter for non “C” mount type cameras (52mm O.D. thread) For TC Series front camera port only: (Requires additional DCA adapter ring for your specific model of camera which are listed on the last two pages of the TC5000 section) CAMERA ADAPTERS FOR 35mm TYPE CAMERAS
   • MA869: 35mm SLR camera attachment Magnification at film plane is 2.23X of objective in use

Stages (Fixed 245mm x 180mm, plain stage is included with stand):
   • MA878: Replacement glass stage plate insert for plain stage - TC5000 Series (Round - 109mm diameter, 1.5mm thick with 44.3mm opening) (Fits plain stage only not for use with MA380/05)
   • MA380/05: Attachable mechanical stage, movement 112mm(X) x 72mm(Y) with test plate holder and drop-down coaxial X-Y controls.

COMPONENTS OFTEN PAIRED WITH THE MA380/05 MECHANICAL STAGE

Specimen Holders for use with MA380/05 Attachable Mechanical Stage (Rectangular Plate 129.74mm x 89.78 mm x 3mm):
   • MA382: Slide glass holder, 1”x 3” 
   • MA383: Chamber holder (For 55mm x 80mm chamber)
   • MA384: Petri dish holder 35mm diameter
   • MA385: Petri dish holder 55mm diameter
   • MA387: Petri dish holder 65mm diameter
   • MA864: Terasaki plate holder (For 56mm x 87mm Terasaki plate)
   • MA378: Hemacytometer holder

Stage Micrometers:
   • MA285: Objective micrometer, 0.01mm ruling
   • MA286: 0.04” divided into 40 units, 0.001
   • MA285: 1mm divided into 100 parts

Eyepiece Micrometers:
   • MA523: Cross-line reticule, 25mm diameter
   • MA506: Eyepiece micrometer, 10mm divided into 100 units, 25mm diameter
   • MA509: Eyepiece micrometer 5mm divided into 100 units, 25mm diameter
   • MA524: 10mm square divided into 100 parts, 1.0mm square, 25mm diameter
   • MA542: Cross-line with 0.1mm graduations, 21mm diameter