Cleaning and Handling Information
Orientation Instructions
Nomenclature and Conventions
Cleaning and Handling
Information
Handle coated pieces by the
edges only. Clean gently only if necessary. Loose
particles should be removed with a bulb puffer or
filtered, pressurized air cleaner. If necessary, gently
wipe surface using anhydrous alcohol and lint-free lab
towels. Use new surface of towel with each wipe.
AVOID TOUCHING OR WIPING A/R COATED OR METAL MIRROR
SURFACES
AVOID HANDLING EXPOSED COATINGS WITH BARE FINGERS
Orientation Instructions
Proper orientation of the
filter is necessary in order to minimize
autofluorescence and maximize performance. There is a
caret (arrow) located on the edge of each filter in
order to aid orientation.
Excitation (x) filters should be positioned with the
arrow pointing toward the specimen, toward the inside of
the cube, and away from the light source.
Emission (m) filters should be placed with the arrow
pointing toward the specimen, toward the inside of the
cube, and away from the detector/eye.
Dichroic mirrors should be mounted with the coated
surface toward the light source, excitation filters, and
the specimen. The dichroics either have an arrow on the
side pointing to the coated side, or they are beveled on
the coated side. The beveled side is the smaller
surface.
Nomenclature and Conventions
Excitation filter (D480/30x)
-- For this example, the center wavelength is at 480nm;
full bandwidth is 30 [ = +/- 15]. In some cases for
which the band width is not specified the letter "x" is
used to define the filter as an excitation filter. This
is generally used for narrow band UV excitation filters,
i.e. d340x.
Dichroic beamsplitter (505DCLP) -- The cut-on wavelength
is approximately 505nm for this dichroic longpass
filter.
Emission filter (D535/40m) -- The center wavelength here
is at 535nm; full bandwidth is 40nm [ = +/- 20].
LP -- indicates a longpass filter which transmits
wavelengths longer than the cut-on and blocks shorter
wavelengths
SP -- indicates a shortpass filter which transmits
wavelengths shorter than the cut-on, and blocks longer
wavelengths
DCLP -- dichroic longpass
DCXR -- dichroic long pass, extended reflection
DCXRU -- dichroic longpass, extended reflection
including the UV
PC -- polychroic beamsplitter. This is a beamsplitter
that reflects and transmits more than two bands of
light.
GG -- Green Glass. Longpass absorption glass from Schott
Glassworks with cut-on wavelengths in the violet and
blue-green regions.
OG -- Orange Glass. Longpass absorption glass from
Schott Glassworks with cut-on wavelengths in the green,
yellow and orange regions.
RG -- Red Glass. Longpass absorption glass from Schott
Glassworks with cut-on wavelengths in the red and far
red regions.
x -- excitation filter
bs -- beamsplitter
m -- emission filter
The list on the left provides the item number of the
filter set along with its component parts and their
spectral characteristics. The graph on the right shows
the spectral trace of this filter set with each of the
components shown in a different color.
References
1. Chroma Technology Corp.
c2008. Cleaning & Handling Information. Available from:
http://www.chroma.com/support/ordering-information/cleaning-handling-information
2. Chroma Technology Corp.
c2008. Orientation Instructions. Available from:
http://www.chroma.com/support/ordering-information/orientation-instructions
3. Chroma Technology Corp.
c2008.Nomenclature and Conventions. Available from:
http://www.chroma.com/support/ordering-information/nomenclature-and-conventions
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